Nucleotide sequence of full length cDNAs encoding rat cardiac myosin light chain-2
نویسندگان
چکیده
منابع مشابه
Heart myosin light chain 2 gene. Nucleotide sequence of full length cDNA and expression in normal and hypertensive rat.
We have isolated and characterized a cDNA recombinant plasmid (pRLC429) specific for the rat heart myosin light chain 2 (MLC2). The cDNA insert consists of 446 base pairs, including a 72-base pair segment of the 3'-untranslated region. Additional 5'-sequence, not present in plasmid pRLC429, was obtained by primer extension of the cDNA. The extended cDNA sequence combined with the plasmid pRLC42...
متن کاملThe nucleotide sequence of a rat myosin light chain 2 gene.
A rat myosin light chain 2 gene was characterized by nucleotide sequence and S1 mapping analyses. It contains seven exons separated by six introns. The corresponding mRNA is predicted to be 654 nucleotides long (excluding polyA sequences), with 5'-nontranslated, coding, and 3'-nontranslated lengths of 56, 510, and 88 nucleotides, respectively. The predicted amino acid sequence is identical to t...
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Pyruvate kinase (PK), a cytosolic enzyme, catalyzes the conversion of phosphoenolpyruvate' to pyruvate with the generation of ATP. We have isolated a full length cDNA clone from a Xgtll cDNA library constructed from rat pituitary GH3 cells by using a 475 bp Pstl fragment from a human PK cDNA (1). The sequence is 2310 bp long and contains a long open reading frame of 1593 bp with an initiation c...
متن کاملIsolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. Sequence and tissue distribution.
A cDNA clone encoding skeletal muscle myosin light chain kinase (MLCK) was isolated from a rat skeletal muscle library using oligonucleotide probes. The total length of the rat skeletal muscle MLCK cDNA was 2823 base pairs with an open reading frame of 1830 base pairs. The deduced sequence of the 610-amino acid protein exhibited 96% amino acid identity to rabbit skeletal muscle MLCK in the carb...
متن کاملDictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the regulatory light chain.
Phosphorylation of the regulatory light chains (RMLC) of nonmuscle myosin can increase the actin-activated ATPase activity and filament formation. Little is known about these regulatory mechanisms and how the RMLC are involved in ATP hydrolysis. To better characterize the nonmuscle RMLC, we isolated cDNAs encoding the Dictyostelium RMLC. Using an antibody specific for the RMLC, we screened a la...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.10.4722